[2025] A Compilation of Anisong Duets [Male-Female / Same-Gender]
Anime is now one of the cultural phenomena Japan can proudly share with the world as part of its pop culture.
The anime songs that color these scenes are no longer just for anime fans! Chances are, your favorite artist or band has also done a theme song for an anime at some point.
In this article, we’ll focus specifically on duet songs among popular anime tracks.
While duets traditionally mean a male-female pair, this time we’ll also include songs by two men and two women.
Feel free to use this as a guide when you’re looking for songs to sing at karaoke!
- [Male and Female] A Compilation of Vocaloid Duet Songs [Also Great for Karaoke]
- Anime Anthem Bangers That Hype Up Karaoke [Gather, Anime Fans!]
- [Collection of Epic Songs] A compilation of cool anime songs chosen by anime fans
- [Burning] A Collection of Hype, Hot Anime Songs
- [Nostalgic & Latest] A Special Feature on Anime Songs Recommended for People in Their 20s
- [Nostalgic] Classic and Popular Anime Songs Recommended for People in Their 30s
- [Latest & Greatest] A Special Feature on Anime Songs Recommended for Teens
- A Special Feature on Easy-to-Sing Anime Songs for Karaoke [100+ Songs]
- Recommended female vocalists for anime songs
- Anime songs recommended for people in their 60s: from nostalgic classics to the latest hits
- Vocaloid Anime Song Compilation [From Opening/Ending Themes to Cover Works]
- [Latest & Timeless] Recommended Anime Songs for Women [All Genres]
- Anime Song Karaoke Popularity Rankings [2025]
Duet Songs from Anime and Character Songs (11–20)
Flying DeityAyase × R-Shitei

Ayase, also known as the composer of YOASOBI.
He collaborated with rapper R-Shitei on the track Hiten.
It serves as the opening theme for the anime Rurouni Kenshin: Meiji Swordsman Romantic Story, with Ayase handling the vocal parts and R-Shitei performing the rap, and the two harmonizing on the chorus.
While not overt, the lyrics are sprinkled with words related to the work, resulting in a song that conveys a deep love for the anime.
Preserved RosesNana Mizuki × T.M.Revolution

That voice gives you chills when you listen to it.
It’s “Preserved Roses” by T.
M.
Revolution and Nana Mizuki.
They captivate in the video as well! It was used as the opening theme for Valvrave the Liberator.
It has a power that’s perfect for an opening!
Absolute, Absolute, Absolute Sacred Domainano feat. ikuta lira

“Zetsu Zetsu Zetsu Absolute Sanctuary” is the theme song for the animated film Dead Dead Demon’s Dededede Destruction.
The duet by ano and Lila Ikuta drew significant attention.
The lyrics center on the resolve to face the end of the world and the bond with a loved one, conveying a message of hope and love found amid chaos.
The single, released on March 20, 2024, also includes the anime edit version and an instrumental version.
It’s a perfect track for immersing yourself in an apocalyptic atmosphere.
StormyNissy × SKY-HI

This is the song that became the theme for the anime film Blue Lock the Movie -Episode Nagi-, created by a collaboration between Nissy and SKY-HI.
Riding a powerful beat, the music—featuring Nissy’s soulful vocals and SKY-HI’s sharp rap—can only be described as breathtaking.
The lyrics convey the clashing emotions of the characters.
The CD released on April 17, 2023, comes in a deluxe edition using the anime’s artwork.
With its dramatic sound, it’s sure to amplify this story of passionate youths striving for the top.
If you want to experience the mindset of rivals pushing each other to the limit, be sure to try singing it at karaoke as well.
O.C. 〜Optimum Combination〜Rei (CV: Yuu Serizawa) & Claire (CV: Karin Nanami)

O.
C.
~Optimum Combination~ has become a hot topic for its unique production.
It’s the ending theme of the anime I’m in Love with the Villainess.
It’s a duet by Yū Serizawa, the voice of Rae, and Karin Nanami, the voice of Claire, and the lead vocal switches between Season 1 and Season 2.
Each version has its own appeal, so be sure to compare them.
It’s also interesting how each version expresses the characters’ personalities in its own way.
The feeling of wanting to knowShy/Momijiyama Teru (CV: Shino Shimoji), Koishikawa Itsuko (CV: Nao Toyama)

The anime SHY portrays the heroes’ exploits and growth.
Its ending theme, “Shiritai Kimochi,” has also become a hot topic.
The song is presented as being sung by the main character, Teru Momijiyama, and her friend Iko Koishikawa, with voice actors Shino Shimoji and Nao Toyama performing.
The lyrics depict the bond and friendship between the two, giving you a warm, cozy feeling when you listen.
Another highlight is the performance style that incorporates playful back-and-forth like a character dialogue.
Give it a listen when you want to be soothed.
Duet Songs from Anime and Character Songs (21–30)
Moonlight SymphoniaAKINO & AIKI from Bless4

Who is the artist behind that beautiful singing voice in the ending of Aquarion EVOL? It’s “Gekkou no Symphonia” sung by the sibling duo AKINO & AIKI.
The first time I heard it, I was enchanted, thinking, “What a beautiful vocal harmony!” It’s a strong track that ranked second in Oricon’s anime category in 2012.






